Western blot analysis of PCDH15 in Lane 1: human placenta tissue lysate, Lane 2: human U2OS whole cell lysate, Lane 3: human HeLa whole cell lysate, Lane 4: rat brain tissue lysate, Lane 5: rat lung tissue lysate, Lane 6: mouse brain tissue lysate, Lane 7: mouse lung tissue lysate, Lane 8: mouse kidney tissue lysate, Lane 9: mouse spleen tissue lysate, Lane 10: mouse Neuro-2a whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with PCDH15 polyclonal antibody (Product # PA5-95603) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).